Image processing for cryo electron microscopy

by biofisica · Published 04/02/2015 · Updated 17/06/2015

EMBO | Practical Course: Image processing for cryo electron microscopy. 1 – 11 September 2015, London (United Kingdom).

Course Web Site: Follow this Link

The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists wishing to determine macromolecular structures by cryo electron microscopy (EM). The course will concentrate on processing of single particle images, and will be aimed at advanced PhD students and postdocs using cryo EM images for structural analysis.

Topics

Basic concepts of Fourier analysis
Principles of TEM image formation and contrast transfer
Image acquisition and pre-processing
Contrast transfer function correction
Symmetry and alignment
Statistical analysis of images
Determination of orientations for 3D reconstruction
Methods of 3D reconstruction
Tomography and subtomogram averaging
Icosahedral reconstruction
Electron crystallography
Refinement and validation of 3D EM reconstructions
Interpretation: Atomic structure fitting into EM maps
Developments in biological electron microscopy: automated data collection
Data deposition

Deadlines

11 May 2015, Registration and Abstract submission

Travel grants

A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event. Selection of awardees is handled directly by the organizer who will notify all eligible participants.