Image processing for cryo electron microscopy
EMBO | Practical Course: Image processing for cryo electron microscopy. 1 – 11 September 2015, London (United Kingdom).
Course Web Site: Follow this Link
The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists wishing to determine macromolecular structures by cryo electron microscopy (EM). The course will concentrate on processing of single particle images, and will be aimed at advanced PhD students and postdocs using cryo EM images for structural analysis.
- Basic concepts of Fourier analysis
- Principles of TEM image formation and contrast transfer
- Image acquisition and pre-processing
- Contrast transfer function correction
- Symmetry and alignment
- Statistical analysis of images
- Determination of orientations for 3D reconstruction
- Methods of 3D reconstruction
- Tomography and subtomogram averaging
- Icosahedral reconstruction
- Electron crystallography
- Refinement and validation of 3D EM reconstructions
- Interpretation: Atomic structure fitting into EM maps
- Developments in biological electron microscopy: automated data collection
- Data deposition
- 11 May 2015, Registration and Abstract submission
A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event. Selection of awardees is handled directly by the organizer who will notify all eligible participants.