Vera…Carrión-Vázquez {Angew Chem 55: 13970}

Protein engineering strategy to directly measure the rupture forces of a protein-protein pair (red) by force spectroscopy, using an atomic force microscope: An elastic “molecular staple” (blue), whose length acts as a fingerprint to identify the rupture event in a recording, is covalently built in parallel to the intermolecular interaction.