Terrazas…Orozco {Nucleic Acids Res 44: 4354}

Rational design of novel N-alkyl-N capped biostable RNA nanostructures for efficient long-term inhibition of gene expression. Nucleic Acids Res. 2016 May 19;44(9):4354-67.
In silico designed N-alkyl-N capped dumbbell-shaped structures were efficiently synthesized by double ligation of BCn-loop hairpins (n = number of carbon atoms of the alkyl chain). The resulting BCn-loop dumbbells displayed strong biostability and efficient RNA interference activity against a range of mRNA targets. The best dumbbell design (containing BC6 loops and 29 bp) was successfully used to silence GRB7 expression in HER2+ breast cancer cells for longer periods of time than natural siRNAs and known biostable dumbbells.