Measuring local-directional resolution and local anisotropy in cryo-EM maps

Measuring local-directional resolution and local anisotropy in cryo-EM maps. Nat Commun. 2020 Jan 2;11(1):55. doi: 10.1038/s41467-019-13742-w.
By incorporating directionality, to the already common local resolution, in different parts of a specimen 3D map, we can estimate how reliable is the local information differentiating among all possible directions. This approach allows estimating the quality of a cryoEM map without the need of original images and opens new ways for model tracing and for sharpening and enhancing the maps.