Latorre…Somoza {Angew Chem 55: 3548}
The activity and selectivity of the gene editing system known as CRISPR/Cas9 has been improved with the introduction of chemical modifications in the RNA strands.
Modified RNAs in CRISPR/Cas9: An Old Trick Works Again
Latorre A, Latorre A, Somoza Á.
Angew Chem Int Ed Engl 2016 Mar.; 55: 3548. [Epub 16 Feb. 2016]
Old tricks, new dog: CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off-target effects.
PubMed: 26880106. Doi: 10.1002/anie.201512002.
