Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion

Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion. eLife. 2017 May 6;6. pii: e24278. doi: 10.7554/eLife.24278.
The diagram fusion assays between proteoliposomes containing synaptobrevin and proteoliposomes containing syntaxin-1-SNAP-25 complex in the presence of NSF, αSNAP, a Munc13-1 fragment and WT or mutant Munc18-1. The D326K mutation in Munc18-1, which enhances synaptobrevin binding, leads to a gain of function, whereas the L348R mutations, which disrupts synaptobrevin binding, impairs the function of munc18-1 in membrane fusion. The M330E mutation does not alter synaptobrevin binding or fusion.