A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

A HaloTag-TEV Genetic Cassette for Mechanical Phenotyping of Proteins From Tissues. Nat Commun . 2020 Apr 28;11(1):2060. doi: 10.1038/s41467-020-15465-9.
The HaloTag-TEV cassette allows bright fluorescent detection of the tagged protein in tissues, as shown here for the giant protein titin in skeletal muscle. The cassette also enables probing protein mechanics in tissues and ex vivo using single-molecule force spectroscopy.